Journal: Journal of cellular and molecular medicine

Article Title: Kindlin-3 Promotes Angiogenesis via Notch Signalling and Is Crucial for Functional Recovery Postmyocardial Infarction.

doi: 10.1111/jcmm.70494

Figure Lengend Snippet: FIGURE 3 | Kindlin-3 promotes angiogenesis in vivo. (A) Representative images of Masson's trichrome staining and quantitative data illustrat- ing the extent of fibrosis in heart sections from the indicated groups (n = 6). Scale bars = 500 μm. (B) Four weeks post-MI, ventricular tissues were analysed using immunofluorescence staining to evaluate capillary (CD31) and arteriolar density (α-SMA) in AAV-NC and AAV-K3 mice post-MI or Sham operation, including the remote area (RA) and border area (BA, n = 6). (C) Representative images and quantitative analysis of Western blots demonstrate the expression of angiogenesis-related proteins in mice 28 days post-MI (n = 3). Data were presented as mean ± SEM. ns, not significant, *p < 0.05, **p＜0.01.

Article Snippet: Name of antibody Manufacturer Identifier Concentration Kindlin- 3 Affinity Biosciences DF13558 1:1000 (WB), 1:200 (IHC) VEGF Proteintech 19003- 1- AP 1:1000 (WB) β1- integrin Bioss bs- 0486R 1:2000 (WB), 1:400 (IHC) GAPDH Proteintech 6004- 1- Ig 1:2000 (WB) Hif- 1α Affinity Biosciences BF8002 1:2000 (WB) eNOS Proteintech 27120- 1- AP 1:1000 (WB) p- eNOS Proteintech 28937- 1- AP 1:1000 (WB) CD31 Proteintech 11265- 1- AP 1:1000 (WB), 1:400 (IF) Cleaved Caspase 3 Cell Signaling Technology 9664 1:1000 (WB) Caspase 3 Cell Signaling Technology 9662 1:1000 (WB) α- Actinin Sigma A7811 1:400 (IF) α- SMA Proteintech 14395- 1- AP 1:400 (IF) vWF Proteintech 66682- 1- Ig 1:400 (IF) Notch1 Proteintech 20687- 1- AP 1:1000 (WB), 1:400 (IHC) Hes1 Affinity Biosciences DF7569 1:1000 (WB) HRP- conjugated a Goat Anti- Rabbit Servicebio GB233303 1:200 (IHC) Goat Anti- Rabbit IgG, Peroxidase Conjugated, H + L Biosharp BL003A 1:10000 (WB) Goat Anti- Mouse IgG, Peroxidase Conjugated, H + L Biosharp BL001A 1:10000 (WB) Alexa Fluor 488- conjugated AffiniPure Goat AntiRabbit IgG (H + L) Jackson 115- 585- 003 1:200 (IF) Alexa Fluor 594- conjugated AffiniPure Goat AntiMouse IgG (H + L) Jackson 115- 585- 003 1:200 (IF) Abbreviations: IF, immunofluorescence; IHC, immunohistochemistry; WB, Western blot. nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jcm m .70494 by IN A SP - N E PA L , W iley O nline L ibrary on [21/03/2025].

Techniques: In Vivo, Staining, Immunofluorescence, Western Blot, Expressing

Journal: Radiation Oncology (London, England)

Article Title: Development of delayed pulmonary toxicities and transcriptional changes in pre-existing interstitial lung disease mice after partial thoracic irradiation

doi: 10.1186/s13014-025-02596-w

Figure Lengend Snippet: The infiltration of immune cells during lung remodeling. ( A ) The protein expressions of α-SMA, F4/80 and CD4 were measured using IHC staining in irradiated and contralateral lung tissues at week 24 post-IR. Semi-quantification of α-SMA ( B ), F4/80 ( C ) and CD4 ( D ) IHC intensity was accessed, and data was showed as the means ± SEM in ten fields per mouse in every group. ** P < 0.01, **** P < 0.0001, ns: no significant. Scale bar = 100 μm, magnifying scale bar = 20 μm

Article Snippet: Slides were blocked with BSA (G5001, Servicebio) and then incubated overnight at 4 °C with primary antibodies anti-α-SMA (1:2000, BM0002, BOSTER, CA, USA), anti-F4/80 (1:5000, Abcam) and anti-CD4 (1:1000, Abcam).

Techniques: Immunohistochemistry, Irradiation